《植物生理学报》 2011, 47(5): 452-458

不同干旱胁迫下欧李光合及叶绿素荧光参数的响应

尹赜鹏¹, 刘雪梅¹, 商志伟¹, 任静², 宋兴舜^1,3,*

1东北林业大学生命科学学院, 哈尔滨150040; 2东北农业大学成栋学院, 哈尔滨150030; 3东北林业大学林木遗传育种与生物 技术教育部重点实验室, 哈尔滨150040

通信作者：宋兴舜；E-mail: sfandi@163.com；Tel: 0451-82191755

摘　要：

采用人为控制土壤含水量的方法对欧李进行轻度和重度干旱的处理, 测定叶片的气体交换和叶绿素荧光参数的日变 化。结果表明, 干旱胁迫下欧李叶片净光合速率、蒸腾速率、水分利用效率、气孔导度、PSII最大光化学效率、光化学量 子效率显著下降, 但胞间CO2浓度、非光化学猝灭系数以及叶黄素循脱环氧化状态(Z+0.5A)/(V+A+Z)和Z含量升高。两干 旱处理植株的影响程度存在差异。这表明在长时间干旱条件下, 欧李叶片光合作用的降低受到气孔与非气孔因素的双重 影响, 叶黄素循环的启动增加了胁迫条件下的热耗散能力以保护光合机构免受干旱胁迫的进一步伤害。

关键词：欧李; 干旱胁迫; 光合参数; 荧光参数; 叶黄素循环

收稿：2010-11-29   修定：2011-04-17

资助：国家自然科学基金(30800876)、黑龙江省自然科学基金 (C201017)、中央高校基本科研业务费专项资金(DL09- CA09)和东北林业大学研究生创新基金(Gram09)。

Response of Photosynthesis and Chlorophy II Fluorescence Parameters to Different Drought Stress in Cerasus humilis Bunge.

YIN Ze-Peng¹, LIU Xue-Mei¹, SHANG Zhi-Wei¹, REN Jing², SONG Xing-Shun^1,3,*

¹College of Life Sciences, Northeast Forestry University, Harbin 150040, China; ²College of Chengdong, Northeast Agricultural University, Harbin 150030, China; ³Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin 150040, China

Corresponding author: SONG Xing-Shun; E-mail: sfandi@163.com; Tel: 0451-82191755

Abstract:

In order to study the light response mechanisms of Cerasus humilis under drought stress, leaf gas exchange and chlorophyll fluorescence parameters were measured with mild and severe drought treatment. The results showed that net photosynthesis rate (Pn), transpiration rate (E), water utilization efficiency (WUE), stomatal conductance (Gs) and maximal photochemical efficiency of PS II (Fv/Fm) decreased significantly in seedlings of C. humilis under drought stress. While the values of intercellular CO₂ concentration (Ci), non-photochemical quenching coefficient (NPQ) and the ratio of Z+0.5A and V+A+Z increased. These results indicated that the decrease of photosynthetic capacity was attributed to both stomatal limitation and non-stomatal limitation under long drought treatment. The capacity of heat dissipation was improved by initiating of the xanthophyll cycle, which resulted in the protection of the photosynthetic apparatus from the damage of drought stress.

Key words: Cerasus humilis; drought stress; photosynthesis parameter; chlorophy II fluorescence parameter; xanthophyll cycle